Photobiomodulation preconditioned diabetic adipose derived stem cells with additional photobiomodulation: an additive approach for enhanced wound healing in diabetic rats with a delayed healing wound

AbstractIn this preclinical investigation, we examined the effects of combining preconditioned diabetic adipose-derived mesenchymal stem cells (AD-MSCs) and photobiomodulation (PBM) on a model of infected ischemic delayed healing wound (injury), (IIDHWM) in rats with type I diabetes (TIDM). During the stages of wound healing, we examined multiple elements such as stereology, macrophage polarization, and the mRNA expression levels of stromal cell-derived factor (SDF)-1 α, vascular endothelial growth factor (VEGF), hypoxia-induced factor 1α (HIF-1α), and basic fibroblast growth factor (bFGF) to evaluate proliferation and inflammation. The rats were grouped into: (1) control group; (2) diabetic-stem cells were transversed into the injury site; (3) diabetic-stem c ells were transversed into the injury site then the injury site exposed to PBM; (4) diabetic stem cells were preconditioned with PBM and implanted into the wound; (5) diabetic stem cells were preconditioned with PBM and transferred into the injury site, then the injury site exposed additional PBM. W hile on both days 4, and 8, there were advanced histological consequences in groups 2–5 than in group 1, we found better results in groups 3–5 than in group 2 (p <  0.05). M1 macrophages in groups 2–5 were lower than in group 1, while groups 3–5 were reduced than in group 2 (p <  0.01). M2 macrophages in groups 2–5 were greater than in group 1, and groups 3–5 were greater than in group 2. (p â‰...
Source: Lasers in Medical Science - Category: Laser Surgery Source Type: research