Genetic diversity of Bartonella rpoB haplotypes in domestic cats from Chile

Comp Immunol Microbiol Infect Dis. 2024 Feb 17;107:102150. doi: 10.1016/j.cimid.2024.102150. Online ahead of print.ABSTRACTThe study aimed to determine the inter and intra-host Bartonella spp. genetic diversity in cats from Chile. 'Seventy-nine cats' blood DNA samples qPCR Bartonella spp. positive were subjected to T-A cloning of Bartonella spp. rpoB partial gene (825 bp), and sequencing by Sanger method. The sequences were submitted to phylogenetic and polymorphism analysis. Thirty-six (45.6%) samples were successfully cloned, generating 118 clones of which 109 showed 99.6%-100% identity with Bartonella henselae whereas 9 showed 99.8-100% identity with Bartonella koehlerae. Haplotype analysis yielded 29 different rpoB-B. henselae haplotypes, one (hap#2) overrepresented in 31 out of 33 cats, and 4 rpoB-B. koehlerae haplotypes, with hap#2 represented in all 3 B. koehlerae infected cats. More than one rpoB -B. henselae and B. koehlerae haplotypes were identified in individual cats, reporting by first time coinfection by different B. henselae/B. koehlerae rpoB variants in cats from Chile.PMID:38401221 | DOI:10.1016/j.cimid.2024.102150
Source: Comparative immunology, microbiology and infectious diseases. - Category: Allergy & Immunology Authors: Source Type: research