Effects of mixed extract from two tropical plants on gut microbiome and metabolome in piglets

In this study, we performed a quantitative analysis of 12 compounds derived fromPiper sarmentosum extract (PSE) and guava leaf extract  (GE). In addition, we investigated the effects of mixed extract (ME) of PSE and GE (1:1) on piglets' gut microbiome and metabolome. A total of 200 piglets (Duroc × Landrace × Large Yorkshire, 21-day-old) were randomly assigned into two groups with five replicates of 20 piglets/pen havi ng the same initial body weight. Piglets were fed a basal diet supplemented with ME at 0 (T0) or 200 mg/kg (T1) for 3 weeks. The quantitation results by ultraperformance liquid chromatography linked to triple-quadrupole tandem mass spectrometry showed that vitexin 2-O-rhamnoside and pellitorine were the greatest abundant among six compounds detected in the PSE. In addition, quercetin, isoquercitrin  and avicularin were found to be the richest of all detected compounds in the GE. Findings on experimental animals indicated that three differential metabolites, comprisingL-alanine, sarcosine  and dihydrofolic acid, in T1 compared with T0 groups, have exactly opposite levels trends in serum and faeces. Moreover, two metabolic pathways (i.e., urea cycle and glutamate metabolism) differed significantly in the serum and faeces of piglets between T0 and T1 (p <  0.05). At the same time, T1 had significantly higher relative abundances ofAgathobacter andAlloprevotella than T0 at genus level (p <  0.05). Correlation analysis revealed that the...
Source: Journal of Animal Physiology and Animal Nutrition - Category: Zoology Authors: Tags: ORIGINAL ARTICLE Source Type: research