Resveratrol and its metabolites elicit neuroprotection via high ‐affinity binding to the laminin receptor at low nanomolar concentrations

In this study, based on the quenching of intrinsic tryptophan fluorescence and molecular docking, we found thattrans-resveratrol, its conjugates (glucuronide and sulfate), and dihydro-resveratrol (intestinal microbial metabolite) bind with high affinities (Kd, 0.2 –2 nm) to the peptide G palindromic sequence (near glycosaminoglycan-binding motif) of the 67-kDa laminin receptor (67LR). Preconditioning with low concentrations (0.01 –10 nm) of these polyphenols, especially resveratrol-glucuronide, protected neuronal cells from death induced by serum withdrawalvia activation of cAMP-mediated signaling pathways. This protection was prevented by a 67LR-blocking antibody, suggesting a role for this cell-surface receptor in neuroprotection by resveratrol metabolites.
Source: FEBS Letters - Category: Biochemistry Authors: Tags: Research Letter Source Type: research