Genes, Vol. 15, Pages 281: SP1 Gene Methylation in Head and Neck Squamous Cell Cancer in HPV-Negative Patients
Genes, Vol. 15, Pages 281: SP1 Gene Methylation in Head and Neck Squamous Cell Cancer in HPV-Negative Patients
Genes doi: 10.3390/genes15030281
Authors:
Enar Jumaniyazova
Anna Aghajanyan
Sergey Kurevlev
Leyla Tskhovrebova
Andrey Makarov
Konstantin Gordon
Anastasiya Lokhonina
Timur Fatkhudinov
There is still much to learn about the epigenetic mechanisms controlling gene expression during carcinogenesis. When researching aberrant DNA methylation, active proliferative tumor cells from head and neck squamous cell cancer (HNSCC) can be used as a model. The aim of the study was to investigate the methylation status of CDKN1, CDKN2A, MYC, Smad3, SP1, and UBC genes in tumor tissue (control-normal tissue) in 50 patients (37 men and 13 women) with HPV-negative HNSCC. Methods: Bisulfite conversion methods and methyl-sensitive analysis of high-resolution melting curves were used to quantify the methylation of genes. In all patients and across various subgroups (tongue carcinoma, laryngeal and other types of carcinomas T2, T3, T4 status; age before and after 50 years; smoking and non-smoking), there are consistent differences in the methylation levels in the SP1 gene in tumor DNA compared to normal. Results: The methylation of the SP1 gene in tumor DNA suppresses its expression, hinders HNSCC cell proliferation regulation, and could be a molecular indicator of malignant cell growth. The study of DNA methylation of various genes involved in carcinogenesis is promising...
Source: Genes - Category: Genetics & Stem Cells Authors: Enar Jumaniyazova Anna Aghajanyan Sergey Kurevlev Leyla Tskhovrebova Andrey Makarov Konstantin Gordon Anastasiya Lokhonina Timur Fatkhudinov Tags: Brief Report Source Type: research
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