Production of ρ-Hydroxyacetophenone by Engineered < em > Escherichia coli < /em > Heterologously Expressing 1-(4-Hydroxyphenyl)-Ethanol Dehydrogenase

J Microbiol Biotechnol. 2023 Dec 8;34(2):1-10. doi: 10.4014/jmb.2310.10019. Online ahead of print.ABSTRACTρ-Hydroxyacetophenone is an important and versatile compound that has been widely used in medicine, cosmetics, new materials, and other fields. At present, there are two ways to obtain ρhydroxyacetophenone. One is to extract it from plants, such as Artemisia capillaris Thunb and Cynanchum otophyllum Schneid, and the other is to synthesize it by using chemical methods. Of these two methods, the second is the main one, although it has problems, such as flammable and explosive reagents, difficult separation of by-products, and harsh reaction conditions. To solve these issues, we adopted genetic engineering in this study to construct engineered Escherichia coli containing Hped gene or EbA309 gene. Whole-cell biotransformation was conducted under the same conditions to select the engineered E. coli with the higher activity. Orthogonal tests were conducted to determine the optimal biotransformation condition of the engineered E. coli. The results showed that the optimal condition was as follows: substrate concentration of 40 mmol/l, IPTG concentration of 0.1 mmol/l, an induction temperature of 25o C, and a transformation temperature of 35oC. Under this condition, the effects of transformation time on the ρ-hydroxyacetophenone concentration and cell growth were further studied. We found that as the transformation time extended, the ρhydroxyacetophenone concentration showed a...
Source: Journal of Microbiology and Biotechnology - Category: Biotechnology Authors: Source Type: research