Gene expression of hemostasis biomarkers following HIIT and RIPC

The objective of this study was to examine how these strategies might affect gene expression of hemostasis biomarkers in middle-aged male Wistar rats. Twenty-four male Wistar rats (12-month-old) were randomly assigned to four groups: high-intensity interval training (HIIT), remote ischemic preconditioning  +HIIT (RIPC+HIIT), sham-HIIT (S-HIIT), sham-RIPC+HIIT (S-RIPC+HIIT). The experimental groups followed specific exercise protocols three times per week. The HIIT involved a 5-min warm-up period at 40–50% vVO2max, followed by 6 sets of 2-min high-intensity exercise at 85 –90% vVO2max, interspersed by 5-min inactive recovery. The session ended with a 5-min cool down at 40 –50% vVO2max. The remote ischemic preconditioning procedure was applied on the left lower limb by using a sphygmomanometer in three 5-min cycles of ischemia (pressure of 220  mm Hg) followed by 5 min of reperfusion (pressure of 0 mm Hg). After 3 weeks, the animals were sacrificed 48 h after their last exercise session, and heart tissue was harvested and analyzed with qRT-PCR method for the tissue factor (TF), plasminogen activator inhibitor (PAI), and tissue plasmi nogen activator t-PA gene expression. Both experimental protocols led to a significant (p <  0.05) decrease in TF and PAI-1 gene expression. However, t-PA was elevated significantly in both RIPC+HIIT and HIIT compared to the sham groups (p <  0.05). Our findings suggest that a 3-week regimen of HIIT and RIPC+HIIT can lead...
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research