Alpha/Gamma-Gliadins are the Superior Target Antigens for Detection of Gluten in Foods by Immunoassay

Gliadins, components of wheat gluten, can elicit severe allergic reactions in sensitive individuals and cause severe illness in celiac disease patients. Thus, reliable detection of wheat in food products is critical. The aim was to develop a sensitive immunoassay for the quantitative measurement of wheat in food products. Here, we have compared the performance of antibodies raised against two candidate immunogens: natural Alpha/gamma-gliadins (Tri a 20-21) and recombinant Omega5-gliadin (Tri a 19) for detection of wheat in food matrices.
Source: Journal of Allergy and Clinical Immunology - Category: Allergy & Immunology Authors: Source Type: research