Viruses, Vol. 16, Pages 211: Safety, Immunogenicity, and Mechanism of a Rotavirus mRNA-LNP Vaccine in Mice

Viruses, Vol. 16, Pages 211: Safety, Immunogenicity, and Mechanism of a Rotavirus mRNA-LNP Vaccine in Mice Viruses doi: 10.3390/v16020211 Authors: Chenxing Lu Yan Li Rong Chen Xiaoqing Hu Qingmei Leng Xiaopeng Song Xiaochen Lin Jun Ye Jinlan Wang Jinmei Li Lida Yao Xianqiong Tang Xiangjun Kuang Guangming Zhang Maosheng Sun Yan Zhou Hongjun Li Rotaviruses (RVs) are a major cause of diarrhea in young children worldwide. The currently available and licensed vaccines contain live attenuated RVs. Optimization of live attenuated RV vaccines or developing non-replicating RV (e.g., mRNA) vaccines is crucial for reducing the morbidity and mortality from RV infections. Herein, a nucleoside-modified mRNA vaccine encapsulated in lipid nanoparticles (LNP) and encoding the VP7 protein from the G1 type of RV was developed. The 5′ untranslated region of an isolated human RV was utilized for the mRNA vaccine. After undergoing quality inspection, the VP7-mRNA vaccine was injected by subcutaneous or intramuscular routes into mice. Mice received three injections in 21 d intervals. IgG antibodies, neutralizing antibodies, cellular immunity, and gene expression from peripheral blood mononuclear cells were evaluated. Significant differences in levels of IgG antibodies were not observed in groups with adjuvant but were observed in groups without adjuvant. The vaccine without adjuvant induced the highest antibody titers after intramuscular injecti...
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research