Isolation, Purification, and Properties of Glycoprotein from Sheep Intestinal Mucous Membrane

A glycoprotein inhibiting the growth of colon cancer cells was isolated from sheep intestinal mucous membrane. The glycoprotein, designated IGP, was purified by a combination of ion-exchange chromatography over DEAE-650M and gel-filtration over HW-55F. Purified IGP had a molecular mass of ~60 kDa and consisted of 68.72 ± 2.10% protein and 32.09 ± 1.52% carbohydrates. The monosaccharide composition of the carbohydrate part consisted of glucose (31.28% of the total carbohydrate content), glucosamine (22.28%) andN-acetylgalactosamine (19.38%), mannose (11.14%), fucose (11.28%), and rhamnose (4.64%). The amino-acid composition of IGP was also determined.
Source: Chemistry of Natural Compounds - Category: Chemistry Source Type: research