Two novel phages, Klebsiella phage GADU21 and Escherichia phage GADU22, from the urine samples of patients with urinary tract infection

AbstractPhages are found in a wide variety of places where bacteria exist including body fluids. The aim of the present study was to isolate phages from the urine samples of patients with urinary tract infection. The 10 urine samples were cultured to isolate bacteria and also used as phage sources against the isolated bacteria. From 10 urine samples with positive cultures, 3 phages were isolated (33%) and two of them were further studied. TheKlebsiella phage GADU21 andEscherichia phage GADU22 phages infectedKlebsiella pneumonia andEscherichia coli, respectively. Among the tested 14 species for host range analysis, theKlebsiella phage GADU21 was able to infect two species which areKlebsiella pneumonia andProteus mirabilis, andEscherichia phage GADU22 was able to infect four species which areShigella flexneri,Shigella sonnei andEscherichia coli. Among different isolates of the indicator bacteria for each phage, GADU21 infected half of the tested 20Klebsiella pneumonia isolates while GADU22 infected 85% of the tested 20E. coli isolates. The genome sizes and GC ratios were 75,968  bp and 44.4%, and 168,023 bp and 35.3% for GADU21 and GADU22, respectively. GADU21 and GADU22 were both lytic and had no antibiotic resistance and virulence genes. GADU21 was homologue withKlebsiella phage vB_KpP_FBKp27 but only 88% of the genome was covered by this phage. The non-covered parts of the GADU21 genome included genes for tail-fiber-proteins and HNH-endonuclease. GADU22 had 94.8% homology ...
Source: Virus Genes - Category: Genetics & Stem Cells Source Type: research