An inulin-type fructan CP-A from < em > Codonopsis pilosula < /em > alleviates TNBS-induced ulcerative colitis based on serum-untargeted metabolomics

Am J Physiol Gastrointest Liver Physiol. 2024 Jan 9. doi: 10.1152/ajpgi.00214.2023. Online ahead of print.ABSTRACTUlcerative colitis (UC) is an inflammatory disease with abdominal pain, diarrhea and bloody stool as the main symptoms. Several studies have confirmed that polysaccharides are effective against UC. It is commonly accepted that the traditional benefits of Radix Codonopsis can be attributed to its polysaccharide contents, and inulin-type fructan CP-A is the main active monomer in the polysaccharide components. Herein, we established a 2, 4, 6-trinitrobenzene sulfonic acid (TNBS)-induced UC rat model and lipopolysaccharide (LPS)-induced colonic epithelial cell model (NCM460) to investigate the effect of CP-A on UC. Untargeted metabolomics studies were conducted to identify differential metabolites using ultra-high performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry (UHPLC-Q-TOF/MS) and enrich metabolic pathways in rat serum. The in vivo assays demonstrated that CP-A reduces colonic macroscopic injury, disease activity index (DAI), histopathological score, interleukin (IL)-8 and tumor necrosis factor-alpha (TNF-α) levels, as well as the expression of intercellular adhesion molecules. On the other hand, increase the IL-10 and transforming growth factor- beta (TGF-β) levels. The in vitro experiments indicated that CP-A treatment could reduce nitric oxide (NO) and IL-1β after LPS stimulation. The metabolomics results suggested that CP-...
Source: Am J Physiol Gastroi... - Category: Gastroenterology Authors: Source Type: research