Metabolic engineering of < em > Caldicellulosiruptor bescii < /em > for 2,3-butanediol production from unpretreated lignocellulosic biomass and metabolic strategies for improving yields and titers

In this study, the thermo-cellulolytic bacterium Caldicellulosiruptor bescii was successfully engineered to produce 2,3-butanediol from cellulose, xylan, and directly from unpretreated switchgrass. Genome-scale metabolic modeling of C. bescii was applied to adjust carbon and redox fluxes to maximize productivity of 2,3-butanediol, thereby revealing bottlenecks that require genetic modifications.PMID:38131671 | DOI:10.1128/aem.01951-23

Source: Applied and Environmental Microbiology - December 22, 2023 Category: Microbiology Authors: Tania N N Tanwee Gina L Lipscomb Jason L Vailionis Ke Zhang Ryan G Bing Hailey C O'Quinn Farris L Poole Ying Zhang Robert M Kelly Michael W W Adams Source Type: research