Methylated MFGE8 Promotes Early Brain Injury After Subarachnoid Hemorrhage and Inhibiting Autophagy of Nerve Cell

ConclusionDNMT1 promotes the methylation of MFGE8 promoter region and downregulates MFGE8 level; restoring MFGE8 downregulates P2X7R, and promotes autophagy by limiting the activation of PI3k/Akt/mTOR, thus exerting a protective effect on brain tissue of SAH mice.Highlights• High expression of DNMT1 and P2X7R and low expression of MFGE8 were observed in SAH mice.• Activation of autophagy reduces EBI after SAH in mice.• DNMT1/MFGE8 reduces autophagy in EBI after SAH in both mouse and cell models.• DNMT1 targets the MFGE8 promoter to upregulate its methylation level.• MFGE8 inhibits P2X7R to activate PI3k/AKT/mTOR pathway and autophagy, thus inhibiting EBI after SAH.
Source: Translational Stroke Research - Category: Neurology Source Type: research