T-independent B-cell effect of agents associated with swine grower-finisher diarrhea

AbstractSwine dysentery, spirochetal colitis, and salmonellosis are production-limiting enteric diseases of global importance to the swine industry. Despite decades of efforts, mitigation of these diseases still relies on antibiotic therapy. A common knowledge gap among the 3 agents is the early B-cell response to infection in pigs. Thus, this study aimed to characterize the porcine B-cell response toBrachyspira hyodysenteriae, Brachyspira hampsonii (virulent and avirulent strains),Brachyspira pilosicoli, andSalmonella Typhimurium, the agents of the syndromes mentioned above. Immortalized porcine B-cell line derived from a crossbred pig with lymphoma were co-incubated for 8  h with each pathogen, as well asE. coli lipopolysaccharide (LPS) and a sham-inoculum (n  = 3/treatment). B-cell viability following treatments was evaluated using trypan blue, and the expression levels of B-cell activation-related genes was profiled using reverse transcription quantitative PCR. OnlyS. Typhimurium and LPS led to increased B-cell mortality.B. pilosicoli downregulated B-lymphocyte antigen (CD19), spleen associated tyrosine Kinase (syk), tyrosine-protein kinase (lyn), and Tumour Necrosis Factor alpha (TNF- α), and elicited no change in immunoglobulin-associated beta (CD79b) and swine leukocyte antigen class II (SLA-DRA) expression levels, when compared to the sham-inoculated group. In contrast, all other treatments significantly upregulated CD79b and stimulated responses in other B-cell...
Source: Veterinary Research Communications - Category: Veterinary Research Source Type: research