Menadione and protocatechuic acid: A drug combination with antitumor effects in murine osteosarcoma cells
This study evaluated the cytotoxic and anticancer effects of the association between menadione (MEN) and protocatechuic acid (PCA) in murine OS cells (UMR-106). The concentrations were 3.12 μM of isolated MEN, 500 μM of isolated PCA, and their associations. We performed cell viability assays, morphology modification analysis, cell migration by the wound-healing method, apoptosis by flow cytometry, reactive oxygen species (ROS) production, gene expression of NOX by RT-qPCR, and degradation of MMP-2 and 9 by zymography. Our results showed that the association of MEN+PCA was more effective in OS cells than the compounds alone. The association decreased cell viability, delayed cell migration, and decreased the expression of NOX-2 and ROS. In addition, the MEN+PCA association induced a slight increase in the apoptotic process. In summary, the association can enhance the compound's antitumor effects and establish a higher selectivity for tumor cells, possibly caused by significant mitochondrial damage and antioxidant properties.PMID:38040223 | DOI:10.1016/j.abb.2023.109840
Source: Archives of Biochemistry and Biophysics - Category: Biochemistry Authors: Kelly Karina Inacio Adriano de Souza Pessoa Cintia Kazuko Tokuhara Ana L ígia Pagnan Mariana Liessa Rovis Sanches Vanessa Svizzero Fakhoury Gabriela Silva Neubern de Oliveira Flavia Amadeu de Oliveira Valdecir Farias Ximenes Rodrigo Cardoso de Oliveira Source Type: research
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