Filifactor alocis enhances survival of Porphyromonas gingivalis W83 in response to H2O2 ‐induced stress

The objective of this study is to further determine ifF. alocis, because of its increased resistance to oxidative stress, can affect the survival of other ‘established’ periodontal pathogens under environmental stress conditions typical of the periodontal pocket. Here, we have shown that via their interaction,F.alocis protectsP. gingivalis W83 under H2O2-induced oxidative stress conditions. Transcriptional profiling of the interaction ofF. alocis andP. gingivalis in the presence of H2O2-induced stress revealed the modulation of several genes, including those with ABC transporter and other cellular functions. The ABC transporter operon (PG0682 –PG0685) ofP. gingivalis was not significant to its enhanced survival when cocultured withF. alocis under H2O2-induced oxidative stress. InF. alocis, one of the most highly up-regulated operons (FA0894 –FA0897) is predicted to encode a putative manganese ABC transporter, which in other bacteria can play an essential role in oxidative stress protection. Collectively, the results may indicate thatF. alocis could likely stabilize the microbial community in the inflammatory microenvironment of the periodontal pocket by reducing the oxidative environment. This strategy could be vital to the survival of other pathogens, such asP. gingivalis, and its ability to adapt and persist in the periodontal pocket.
Source: Molecular Oral Microbiology - Category: Microbiology Authors: Tags: ORIGINAL ARTICLE Source Type: research