A potent neutralizing nanobody targeting a unique epitope on the receptor-binding domain of SARS-CoV-2 spike protein

In this study, we reported the identification and structural biological characterization of a nanobody from a RBD-immunized alpaca. The nanobody, termed as 2S-1-19, shows outstanding neutralizing activity against both pseudotyped and authentic SARS-CoV-2 viruses. The crystal structure of 2S-1-19 bound to SARS-CoV-2 RBD reveals an epitope that overlaps with the binding site for ACE2. We also showed that 2S-1-19 reserves promising, though compromised, neutralizing activity against the Delta variant and that the trivalent form of 2S-1-19 remarkably increases its neutralizing capacity. Despite this, neither the monomeric or trimeric 2S-1-19 could neutralize the Omicron BA.1.1 variant, possibility due to the E484A and Q493K mutations found within this virus variant. These data provide insights into immune evasion caused by SARS-CoV-2 variants.PMID:37984151 | DOI:10.1016/j.virol.2023.109925

Source: Virology - November 20, 2023 Category: Virology Authors: Yuting Zhang Dan Wang Qi Xiang Xiaohui Hu Yuting Zhang Lijie Wu Zhaoyong Zhang Yanqun Wang Jincun Zhao Peter J McCormick Jinheng Fu Yang Fu Jin Zhang Haihai Jiang Jian Li Source Type: research