Cytotoxic and genotoxic effects of triphenyl phosphate on root tip cells of Allium cepa L

In this study, we evaluated genotoxicity and cytotoxicity of TPhP on Allium cepa on the cells of root tips. A 10 mg/mL stock solution of TPhP was dissolved in DMSO and dilutions of 2, 4, 6, 8 and 10 mg/mL was made in distilled water. Onion bulbs, rooted in test tubes, were exposed to these concentrations of TPhP for 24 h. Distilled water was used as a negative control, 0.7% hydrogen peroxide was used as positive control, and 0.5% DMSO was used as solvent control. Significant inhibition of onion root growth was observed following treatment with the 6, 8 and 10 mg/mL TPhP ranges in comparison with the negative and solvent control groups (p < 0.05). Furthermore, in the TPhP treatment groups, total chromosome aberration ratios were significantly high in comparison with the controls (p < 0.05). These results suggest that TPhP have cytotoxic and genotoxic effects on A. cepa root tips.PMID:37981031 | DOI:10.1016/j.tiv.2023.105734
Source: Toxicology in Vitro - Category: Toxicology Authors: Source Type: research