TPE conjugated islet amyloid polypeptide probe for detection of peptide oligomers

In this study, we successfully grafted an aggregation-induced emission molecule, tetraphenylethylene (TPE), at the N-terminus of IAPP. By mixing a small amount of TPE-labeled IAPP with unlabeled IAPP, we were able to detect an increase in TPE fluorescence during the nucleation phase of IAPP aggregation in vitro. It may enable real-time monitoring of IAPP oligomer formation and is further applied in the diagnosis of T2D.PMID:37948788 | DOI:10.1016/j.bpc.2023.107129
Source: Biophysical Chemistry - Category: Chemistry Authors: Source Type: research