Δ3-tubulin impairs mitotic spindle morphology and increases nuclear size in pancreatic cancer cells

In this study, we investigated the impacts of forced expression of Δ3-tubulin on the structure of spindle bodies and cell division in a pancreatic cancer cell line, PANC-1. Overexpression of HA-tagged Δ3-tubulin impaired the morphology and orientation of spindle bodies during cell division in PANC-1 cells. In particular, spindle bending was most significantly increased. Expression of EGFP-tagged Δ3-tubulin driven by the endogenous promoter of humanTUBA1B also deformed and misoriented spindle bodies. Spindle bending and condensation defects were significantly observed by EGFP- Δ3-tubulin expression. Furthermore, EGFP-Δ3-tubulin expression increased the nuclear size in a dose-dependent manner of EGFP-Δ3-tubulin expression. The expression of EGFP-Δ3-tubulin tended to slow down cell proliferation. Taken together, our results demonstrate that Δ3-tubulin affects the spind le integrity and cell division.
Source: Medical Molecular Morphology - Category: Molecular Biology Source Type: research