Proficiency tests to evaluate the impact on assay outcomes of harmonized influenza-specific intracellular cytokine staining (ICS) and IFN- ɣ enzyme-linked ImmunoSpot (ELISpot) protocols

In conclusion, proficiency tests show that a limited harmonization effort consisting of the introduction of SOPs and the use of the same in vitro stimulating antigens leads to a reduction of the interlaboratory variation of IFN-ɣ ELISpot data and demonstrate that substantial improvements for the ICS assay are achieved as comparable laboratory datasets could be generated. Additional steps to further reduce the interlaboratory variation of ICS data can consist of standardized gating templates and detailed data reporting instructions as well as further efforts to harmonize reagent and instrument use.PMID:37918618 | DOI:10.1016/j.jim.2023.113584

Source: Journal of Immunological Methods - November 2, 2023 Category: Allergy & Immunology Authors: Gwenn Waerlop Geert Leroux-Roels Anke Pagnon Sarah Begue Bruno Salaun Michel Janssens Donata Medaglini Elena Pettini Emanuele Montomoli Elena Gianchecchi Teresa Lambe Leila Godfrey Maireid Bull Duncan Bellamy H åkon Amdam Geir Bredholt Rebecca Jane Cox F Source Type: research