67. Target capture NGS for use in molecular-based research of myeloid measurable residual disease

Molecular technologies incorporating NGS are increasingly utilised to support traditional immunophenotypic multiparameter flow cytometry in MRD detection, including acute myeloid leukaemia (AML) disease monitoring.We used the OGTTM Universal NGS Complete Workflow Solution library preparation kit and a focussed SureSeqTM Myeloid MRD panel to assess the applicability of a capture-based target enrichment NGS approach for studies of molecular-based MRD monitoring in AML. The panel covers 43 hotspot exons in 13 genes relevant to the AML panel (11.3 kb baited, 8 kb targeted).
Source: Cancer Genetics and Cytogenetics - Category: Genetics & Stem Cells Authors: Source Type: research