The benefit of using < em > in vitro < /em > bioassays to screen agricultural samples for oxidative stress: South Africa's case

J Environ Sci Health B. 2023 Oct 9:1-15. doi: 10.1080/03601234.2023.2264739. Online ahead of print.ABSTRACTApplied pesticides end up in non-target environments as complex mixtures. When bioavailable, these chemicals pose a threat to living organisms and can induce oxidative stress (OS). In this article, attention is paid to OS and the physiological role of the antioxidant defense system. South African and international literature was reviewed to provide extensive evidence of pesticide-induced OS in non-target organisms, in vivo and in vitro. Although in vitro approaches are used internationally, South African studies have only used in vivo methods. Considering ethical implications, the authors support the use of in vitro bioassays to screen environmental matrices for their OS potential. Since OS responses are initiated and measurable at lower cellular concentrations compared to other toxicity endpoints, in vitro OS bioassays could be used as an early warning sign for the presence of chemical mixtures in non-target environments. Areas of concern in the country could be identified and prioritized without using animal models. The authors conclude that it will be worthwhile for South Africa to include in vitro OS bioassays as part of a battery of tests to screen environmental matrices for biological effects. This will facilitate the development and implementation of biomonitoring programs to safeguard the South African environment.PMID:37814453 | DOI:10.1080/03601234.2023.2264739
Source: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes - Category: Environmental Health Authors: Source Type: research