Dose Dependent Effect of Sulfamethoxazole on Inhibiting K < sub > ATP < /sub > Channel of Mouse Pancreatic β Cell

In this study, whole-cell patch clamp recording was utilized to measure the effect of SMX on KATP channel activity on pancreatic β cells. Also, the static incubation assay with mice islets was assessed to measure the insulin secretion capacity of SMX. SMX was shown to inhibit the KATP channel in pancreatic β cell membrane and induce insulin secretion in relatively high concentration. The half maximal inhibitory concentration (IC50) for KATP channel activity of SMX was .46 ± .08 mM. It was also shown that a near IC50 concentration of SMX (.5 mM) was able to nearly fully block the KATP channel when simultaneously applied with low concentration sulfonylurea, tolbutamide (.01 mM). Our present data provide important information for the clinical use of SMX to treat infection in diabetic patients using sulfonylureas.PMID:37780606 | PMC:PMC10540586 | DOI:10.1177/15593258231203611

Source: Dose Response - October 2, 2023 Category: Drugs & Pharmacology Authors: Hiroshi Ogata Shigeki Kitamura Makoto Fujiwara Masaru Shimizu Chengbo Tan Songji Zhao Yuko Maejima Kenju Shimomura Source Type: research