The ester production capacity of Pichia kudriavzevii based on functional annotation of genes

This study focused on the yeast strainPichia kudriavzevii, renowned for its high capacity to produce esters. Whole genome sequences were annotated and analyzed using the GO, KEGG, KOG, CAZy, and Pfam databases to determine the genetic basis underly the enhanced ester production capacity. Results showed thatP. kudriavzevii gene function was concentrated in biosynthetic capacity, metabolic capacity, amino acid translocation capacity, glycoside hydrolysis capacity and transfer capacity. Additionally, acyltransferase and kinase were predicted as active sites contributing toP. kudriavzevii high ester production. We further compared the volatile composition differences betweenP. kudriavzevii andSaccharomyces cerevisiae through Headspace solid-phase microextraction-gas Chromatography-mass spectrometry (HS-SPME-GC-MS), revealingP. kudriavzevii produced 3.5 times more esters thanS. cerevisiae. Overall, our findings suggest thatP. kudriavzevii had potential applications in the Baijiu brewing industry.
Source: World Journal of Microbiology and Biotechnology - Category: Microbiology Source Type: research