Gut microbiota contribution to selenium deficiency ‐induced gut–liver inflammation

Se deficient imbalance the intestinal microbiota. Se deficient effects gut –liver axis via lipopolysaccharide.Lactobacillus reuteri could alleviate selenium deficiency-induced gut –liver axis injury. AbstractThere is limited knowledge about the factors that drive gut –liver axis changes after selenium (Se) deficiency-induced gut or liver injuries. Thus, we tested Se deficiency in mice to determine its effects on intestinal bacterial balance and whether it induced liver injury. Serum Se concentration, lipopolysaccharide (LPS) level, and liver injury biomarkers were tested using a biochemical method, while pathological changes in the liver and jejunum were observed via hematoxylin and eosin stain, and a fluorescence spectrophotometer was used to evaluate intestinal permeability. Tight junction (TJ)-related and toll-like receptor (TLR) signaling-related pat hway genes and proteins were tested using quantitative polymerase chain reaction, western blotting, immunohistochemistry, and 16S ribosomal ribonucleic acid gene-targeted sequencing of jejunum microorganisms. Se deficiency significantly decreased glutathione peroxidase activity and disrupted the int estinal flora, with the most significant effect being a decrease inLactobacillus reuteri. The expression of TJ-related genes and proteins decreased significantly with increased treatment time, whereas supplementation with Se, fecal microbiota transplantation, orL. reuteri reversed these decreases. Signs of liver injury and L...
Source: BioFactors - Category: Biochemistry Authors: Tags: RESEARCH ARTICLE Source Type: research