Rhabdovirus encoded glycoprotein induces and harnesses host antiviral autophagy for maintaining its compatible infection

Autophagy. 2023 Sep 1:1-20. doi: 10.1080/15548627.2023.2252273. Online ahead of print.ABSTRACTMacroautophagy/autophagy has been recognized as a central antiviral defense mechanism in plant, which involves complex interactions between viral proteins and host factors. Rhabdoviruses are single-stranded RNA viruses, and the infection causes serious harm to public health, livestock, and crop production. However, little is known about the role of autophagy in the defense against rhabdovirus infection by plant. In this work, we showed that Rice stripe mosaic cytorhabdovirus(RSMV) activated autophagy in plants and that autophagy served as an indispensable defense mechanism during RSMV infection. We identified RSMV glycoprotein as an autophagy inducer that interacted with OsSnRK1B and promoted the kinase activity of OsSnRK1B on OsATG6b. RSMV glycoprotein was toxic to rice cells and its targeted degradation by OsATG6b-mediated autophagy was essential to restrict the viral titer in plants. Importantly, SnRK1-glycoprotein and ATG6-glycoprotein interactions were well-conserved between several other rhabdoviruses and plants. Together, our data support a model that SnRK1 senses rhabdovirus glycoprotein for autophagy initiation, while ATG6 mediates targeted degradation of viral glycoprotein. This conserved mechanism ensures compatible infection by limiting the toxicity of viral glycoprotein and restricting the infection of rhabdoviruses.Abbreviations: AMPK: adenosine 5'-monophosphate (AMP)-a...

Source: Autophagy - September 1, 2023 Category: Cytology Authors: Xiuqin Huang Junkai Wang Siping Chen Siying Liu Zhanbiao Li Zhiyi Wang Biao Chen Chong Zhang Yifei Zhang Jinhui Wu Xiaorong Yang Qingjun Xie Faqiang Li Hong An Jilei Huang Huali Li Chuanhe Liu Xiaoxian Wu Ding Xiang Liu Xin Yang Guohui Zhou Tong Zhang Source Type: research