Assessment of cellular damage with cytome assay among environmental/occupational triazole

Chem Biol Interact. 2023 Aug 28:110689. doi: 10.1016/j.cbi.2023.110689. Online ahead of print.ABSTRACTThe use of triazole fungicides is common in Minas Gerais, Brazil. However, the risk arising from excessive and often unprotected exposure can be harmful to farmers. Therefore, we evaluated volunteers, exposed to triazole fungicides for cellular damage caused by this pesticide. In the buccal micronucleus cytome assay (BMCyt), cells were analyzed. Urinary triazoles were analyzed by the Liquid-Liquid Extraction coupled with Gas-chromatography/mass-spectrometry (LLE-GC/MS). Statistical differences were found for all cell types evaluated in residents of rural areas (n = 145). Analysis of variance showed statistical difference in kariolytic and pyknotic cells, between the groups of men and women living in rural areas, with higher incidence in the male group. Likewise, higher concentrations triazoles in urine samples in the male group were observed. Greater cellular damage suggests increases in DNA damage, chromossomal instability and cell deaths. The results showed the urgency of the public management with the implementation of measures to minimize the pesticides exposure.PMID:37648053 | DOI:10.1016/j.cbi.2023.110689

Source: Chemico-Biological Interactions - August 30, 2023 Category: Molecular Biology Authors: Luiz F Costa Luiz P A Marciano Fernando Feltrim Josiane O Freire Gislaine B Silva Alessandra C P Silv ério Isarita Martins Source Type: research