Binding Affinity Studies of Nicotinamide N-methyltransferase and Ligands by Saturation Transfer Difference NMR

CONCLUSION: The binding affinity of nicotinamide and SAH to NNMT and its mutants were obtained by STD NMR in this study. It was found that nicotinamide and SAH bind to WT in a particular orientation, and Y20 is critical for their binding orientation and affinity to NNMT.PMID:37622714 | DOI:10.2174/0929866530666230824153356
Source: Protein and Peptide Letters - Category: Biochemistry Authors: Source Type: research