Structural perspectives on transcription in chromatin

Trends Cell Biol. 2023 Aug 16:S0962-8924(23)00155-1. doi: 10.1016/j.tcb.2023.07.011. Online ahead of print.ABSTRACTIn eukaryotes, all genetic processes take place in the cell nucleus, where DNA is packaged as chromatin in 'beads-on-a-string' nucleosome arrays. RNA polymerase II (RNAPII) transcribes protein-coding and many non-coding genes in this chromatin environment. RNAPII elongates RNA while passing through multiple nucleosomes and maintaining the integrity of the chromatin structure. Recent structural studies have shed light on the detailed mechanisms of this process, including how transcribing RNAPII progresses through a nucleosome and reassembles it afterwards, and how transcription elongation factors, chromatin remodelers, and histone chaperones participate in these processes. Other studies have also illuminated the crucial role of nucleosomes in preinitiation complex assembly and transcription initiation. In this review we outline these advances and discuss future perspectives.PMID:37596139 | DOI:10.1016/j.tcb.2023.07.011
Source: Trends in Cell Biology - Category: Cytology Authors: Source Type: research