Understanding the Molecular Basis for Enhanced Glutenase Activity of Actinidin using Structural Bioinformatics

In this study, we aimed to understand the structural basis for the elevated protease action of actinidin against gliadin peptides by using an in silico approach. Docking experiments revealed key differences between the binding of gliadin peptide to actinidin and papain, which may be responsible for their differential digestive action. Sequence comparison of different plant cysteine proteases highlights amino acid residues surrounding the active site pocket of actinidin that are unique to this molecule and hence likely to contribute to its digestive properties.PMID:37592795 | DOI:10.2174/0929866530666230817141100
Source: Protein and Peptide Letters - Category: Biochemistry Authors: Source Type: research