Generation of a novel < em > ex-vivo < /em > model to study re-endothelialization

Artif Cells Nanomed Biotechnol. 2023 Dec;51(1):408-416. doi: 10.1080/21691401.2023.2245456.ABSTRACTEndothelial dysfunction initiates the pathogenesis of a myriad of cardiovascular diseases, yet the precise underlying mechanisms remain unclear. Current model utilises mechanical denudation of arteries resulting in an arterial-injury model with onset of intimal hyperplasia (IH). Our study shows that 5 min enzymatic denudation of human umbilical artery (hUA) lumen at 37 °C efficiently denudes hUA while maintaining vessel integrity without significantly increase intima-media thickness after 7 days in culture. This ex-vivo model will be a valuable tool in understanding the mechanism of re-endothelialization prior to smooth muscle cells (SMC) activation thus placating IH at an early stage.PMID:37584645 | DOI:10.1080/21691401.2023.2245456

Source: Artificial Cells, Nanomedicine and Biotechnology - August 16, 2023 Category: Biotechnology Authors: Siti Sarah Azman Muhammad Dain Yazid Nur Azurah Abdul Ghani Raja Zahratul Azma Raja Sabudin Mohd Ramzisham Abdul Rahman Nadiah Sulaiman Source Type: research