Bisphenol A toxicity induced hepatotoxicity and altered biochemical, histopathology, and immunohistochemical parameters: the metal chelating and antioxidant roles of naringin

This study was carried out to assess the potential ameliorative effect of naringin on BPA-induced hepatotoxicity in cockerel chicks. Thirty-one-day-old cockerel chicks were used for this study and randomly divided into 6 groups of five chicks per group as follows: Group 1 (control), Group 2 (BPA 100  ppm), Group 3 (BPA + 100 mg/kg naringin), Groups 4 (BPA + 200 mg/kg naringin), Group 5 (100 mg/kg naringin), and Group 6 (200 mg/kg naringin), respectively. The administration of BPA was via drinking water and naringin through oral gavage. BPA intoxication caused significant (p <  0.05) increases in ALT, ALP, AST, TC, TAG, and LDL, but decrease total protein and HDL-cholesterol when compared with the control. Also, there were significant increases in hepatic H2O2 generation and MDA contents with concomitant decrease in reduced glutathione content, glutathione S-transferase, and superoxide dismutase activity in BPA intoxicated chicks. Histology revealed a moderate to diffuse sinusoidal congestion, with a severe periportal cellular infiltration in BPA intoxicated chicks. Immunohistochemistry revealed higher expression of hepatic caspase 3 and TNF- α in chicks exposed to BPA alone relative to the control and naringin treated chicks (100 mg/kg and 200 mg/kg). Findings from this study show that naringin administration restored hepatoxicity, improved antioxidant status, and lowered exaggerated values of total cholesterol, oxidative stress indi ces, inflammation, ...
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research