Phosphoregulation of the checkpoint kinase Mec1 < sup > ATR < /sup >

DNA Repair (Amst). 2023 Jul 17;129:103543. doi: 10.1016/j.dnarep.2023.103543. Online ahead of print.ABSTRACTYeast Mec1, and its mammalian ortholog, Ataxia-Telangiectasia and Rad3-related, are giant protein kinases central to replication stress and double strand DNA break repair. Mec1ATR, in complex with Ddc2ATRIP, is a 'sensor' of single stranded DNA, and phosphorylates numerous cell cycle and DNA repair factors to enforce cell cycle arrest and facilitate repair. Over the last several years, new techniques - particularly in structural biology - have provided molecular mechanisms for Mec1ATR function. It is becoming increasingly clear how post-translational modification of Mec1ATR and its interaction partners modulates the DNA damage checkpoint. In this review, we summarise the most recent work unravelling Mec1ATR function in the DNA damage checkpoint and provide a molecular context for its regulation by phosphorylation.PMID:37480741 | DOI:10.1016/j.dnarep.2023.103543

Source: DNA Repair - July 22, 2023 Category: Genetics & Stem Cells Authors: Luke A Yates Xiaodong Zhang Source Type: research