Spectroscopic determination of hydrophobic adulterant tadalafil by aptasensor based ellipsometry

In this study, we propose an aptasensor based on ellipsometry for the detection of PDE5 inhibitors. To enhance the detection specificity for PDE5 inhibitors, we designed an aptamer with a hydrophobic pocket that incorporates a guanine base-rich region and a three-way junction. This design is particularly effective considering the poor aqueous solubility of PDE5 inhibitors. Preliminary results demonstrate that tadalafil detection in various media can be achieved within the range of 1-2000 ng/mL. The limit of detection for the active compound of tadalafil is as low as 1.82 ng/mL.PMID:37454513 | DOI:10.1016/j.talanta.2023.124940
Source: Talanta - Category: Chemistry Authors: Source Type: research