Development of a new multiplex quantitative PCR for the detection of Glaesserella parasuis, Mycoplasma hyorhinis, and Mycoplasma hyosynoviae

The aim of the study was to develop a multiplex PCR for identification ofGlaesserella parasuis and its virulence markervtaA distinguishing between highly virulent and non-virulent strains. Furthermore,Mycoplasma hyorhinis andMycoplasma hyosynoviae can be identified targeting 16S rRNA. The developed multiplex PCR provides a simple one-tube assay, which can be performed rapidly and cost-efficiently allowing an efficient throughput of samples in veterinary diagnostic laboratories. AbstractGlaesserella parasuis,Mycoplasma hyorhinis, andMycoplasma hyosynoviae are important porcine pathogens responsible for polyserositis, polyarthritis, meningitis, pneumonia, and septicemia causing significant economic losses in the swine industry. A new multiplex quantitative polymerase chain reaction (qPCR) was designed on one hand for the detection ofG. parasuis and the virulence markervtaA to distinguish between highly virulent and non-virulent strains. On the other hand, fluorescent probes were established for the detection and identification of bothM. hyorhinis andM. hyosynoviae targeting 16S ribosomal RNA genes. The development of the qPCR was based on reference strains of 15 known serovars ofG. parasuis, as well as on the type strainsM. hyorhinis ATCC 17981T andM. hyosynoviae NCTC 10167T. The new qPCR was further evaluated using 21G. parasuis, 26M. hyorhinis, and 3  M. hyosynoviae field isolates. Moreover, a pilot study including different clinical specimens of 42 diseased pigs was perform...
Source: MicrobiologyOpen - Category: Microbiology Authors: Tags: COMMENTARY Source Type: research