A novel [math]-ketoamide reactivity-based two-photon fluorogenic probe for visualizing peroxynitrite in Parkinson ’s disease models

Journal of Innovative Optical Health Sciences, Ahead of Print. Peroxynitrite (ONOO[math] contributes to oxidative stress and neurodegeneration in Parkinson ’s disease (PD). Developing a peroxynitrite probe would enable in situ visualization of the overwhelming ONOO− flux and understanding of the ONOO− stress-induced neuropathology of PD. Herein, a novel [math]-ketoamide-based fluorogenic probe (DFlu) was designed for ONOO− monitoring in multip le PD models. The results demonstrated that DFlu exhibits a fluorescence turn-on response to ONOO− with high specificity and sensitivity. The efficacy of DFlu for intracellular ONOO− imaging was demonstrated systematically. The results showed that DFlu can successfully visualize endogenous and e xogenous ONOO− in cells derived from chemical and biochemical routes. More importantly, the two-photon excitation ability of DFlu has been well demonstrated by monitoring exogenous/endogenous ONOO− production and scavenging in live zebrafish PD models. This work provides a reliable and promising [math]-ketoamide-based optical tool for identifying variations of ONOO− in PD models.
Source: Journal of Innovative Optical Health Sciences - Category: Biomedical Science Authors: Source Type: research