RNA silencing of GM-CSF in CAR-T cells reduces the secretion of multiple inflammatory cytokines

In this study, we interrupted GM-CSF expression in CAR-T cells by inserting the GM-CSF shRNA-expression cassette in the CAR vector. Reduction of GM-CSF in CAR-T cells could decrease the level of several proinflammatory cytokines without hampering the killing capacity. The manufacture of GM-CSF knockdown CAR-T cells does not require complicated transfections, which makes it more practical and feasible for clinical application.
Source: Investigational New Drugs - Category: Drugs & Pharmacology Source Type: research