Diagnostic value of MDM2 RNA in situ hybridization for low-grade osteosarcoma: Consistency comparison of RNA in situ hybridization, fluorescence in situ hybridization, and immunohistochemistry

In conclusion,MDM2 RNA-ISH has great value for the diagnosis of LGOS, with excellent consistency with FISH and better sensitivity than IHC. Acid decalcification still has an adverse impact on RNA. SomeMDM2-nonamplified tumors may show positivity forMDM2 RNA-ISH, which needs to be analyzed comprehensively in combination with clinicopathological features.

http://link.springer.com/10.1007/s00428-023-03530-9

Source: Virchows Archiv - March 28, 2023 Category: Pathology Source Type: research