Comparative analysis of the functional properties of human and mouse ferroportin

We examined the functional properties of human Fpn, mouse Fpn, and mutant mouse Fpn by using radiotracer assays in RNA-injected Xenopus oocytes. We found that neither mouse nor human Fpn transports manganese. Mouse and human Fpn share identical properties with respect to substrate profile, calcium dependence, optimal pH, and hepcidin sensitivity. We have also demonstrated that Fpn is not an ATPase pump. Our findings validate the use of mouse models of ferroportin function in iron homeostasis and disease.PMID:36939203 | DOI:10.1152/ajpcell.00063.2023
Source: Am J Physiol Cell Ph... - Category: Cytology Authors: Source Type: research