Isolation of potent antileishmanial agents from Artemisia kermanensis Podlech using bioguided fractionation

This study aimed to evaluate the secondary metabolites fromArtemisia kermanensis Podlech by bioguided fractionation againstLeishmania major. The chemical structures of the isolated compounds were determined based on analysis of mass and nuclear magnetic resonance spectra. Antileishmanial activity were determined on promastigotes and amastigotes. Chemical structures of the isolated compound were as 1-Acetoxy-3,7-dimethyl-7-hydroxy-octa-2E,5E-dien-4-one for compound 1 and 5,7-dihydroxy-3 ′,4′,6-trimethoxyflavone (Eupatilin) for compound 2, and 5,7,3′-Trihydroxy-6,4′,5′-trimethoxyflavone for compound 3. Compound 2 were confirmed by significant activity with IC50 of less than 50  μg/ml for 24 and 48 h in clinical form (amastigotes). Compound 3 demonstrated high susceptibility with an IC50 of less than 30  μg/ml for promastigotes for 24 h. The bioguided fractionation ofA. kermanensis resulted the isolation of potent antileishmanial agents with a low toxicity effect on macrophages. These plant metabolites can be a candidate as a drug for treating cutaneous leishmaniasis.
Source: Journal of Parasitic Diseases - Category: Parasitology Source Type: research