Knock down of TIMP-2 by siRNA and CRISPR/Cas9 mediates diverse cellular reprogramming of metastasis and chemosensitivity in ovarian cancer

CONCLUSIONS: Our results suggest that the inhibition of TIMP-2 by siRNA and CRISPR/Cas-9 modulate the expression of MMP-2 and MMP-14 and reprogram ovarian cancer cells to facilitate proliferation and invasion. Distinct disparities in in vitro chemosensitivity and growth in 3D culture, and differences in tumour burden and invasion to proximal organs in a mouse model imply that selective suppression of TIMP-2 expression by siRNA or CRISPR/Cas-9 alters important aspects of metastasis and chemosensitivity in ovarian cancer.PMID:36585738 | DOI:10.1186/s12935-022-02838-x

Source: Cancer Control - December 30, 2022 Category: Cancer & Oncology Authors: Ruth M Escalona Simon Chu Elif Kadife Jason K Kelly George Kannourakis Jock K Findlay Nuzhat Ahmed Source Type: research