CANTRK: A Canadian Ring Study to Optimize Detection of NTRK Gene Fusions by Next Generation RNA Sequencing

CANTRK is a ring study to optimize testing for neurotrophic receptor tyrosine kinase (NTRK) fusions in Canadian laboratories. Sixteen diagnostic laboratories used next generation sequencing (NGS) for NTRK1, NTRK2 or NTRK3 fusions. Each laboratory received 12 formalin fixed, paraffin embedded (FFPE) tumor samples with unique NTRK fusions, and 2 control non-NTRK fusion samples (1 ALK and 1 ROS1). Laboratories used validated protocols for NGS fusion detection. Panels included Oncomine Comprehensive Assay v3, Oncomine Focus, Oncomine Precision, AmpliSeq Focus, TruSight RNA Pan-Cancer Panel, FusionPlex Lung and QIAseq Multimodal Lung.

Source: Journal of Molecular Diagnostics - December 28, 2022 Category: Pathology Authors: Tracy L. Stockley, Bryan Lo, Adrian Box, Andrea Gomez Corredor, John DeCoteau, Patrice Desmeules, Harriet Feilotter, Daria Grafodatskaya, Wenda Greer, Cynthia Hawkins, Weei Yuarn Huang, Iyare Izevbaye, Guylaine L épine, Sebastiao N. Martins Filho, Andrea Tags: Regular Article Source Type: research