A next generation targeted amplicon sequencing method to screen for insecticide resistance mutations in < i > Aedes aegypti < /i > populations reveals a < i > rdl < /i > mutation in mosquitoes from Cabo Verde

by Emma L. Collins, Jody E. Phelan, Magdalena Hubner, Anton Spadar, Monica Campos, Daniel Ward, Holly Acford-Palmer, Ana Rita Gomes, Keily Silva, Lara Ferrero Gomez, Taane G. Clark, Susana CampinoAedes mosquito vectors transmit many viruses of global health concern, including dengue, chikungunya and Zika. These vector-borne viral diseases have a limited number of treatment options, and vaccines vary in their effectiveness. Consequently, integrated vector management is a primary strategy for disease control. However, the increasing emergence and spread of insecticide resistance is threatening the efficacy of vector control methods. Identifying mutations associated with resistance in vector populations is important to monitor the occurrence and evolution of insecticide resistance and inform control strategies. Rapid and cost-effective genome sequencing approaches are urgently needed. Here we present an adaptable targeted amplicon approach for cost-effective implementation within next generation sequencing platforms. This approach can identify single nucleotide polymorphisms (SNPs) and small insertions and deletions (indels) in genes involved in insecticide resistance inAedes aegypti mosquitoes. We designed and tested eleven amplicons, which included segments of theace-1 (carbamate target), theVoltage-Gated Sodium Channel (vgsc; pyrethroids, DDT and organochlorines), andrdl (dieldrin) genes; thereby covering established knockdown resistance (kdr) mutations (e.g., S989P, I1011M/V...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research