Novel strategies for genetic manipulation of human iPSC-derived organoid platforms

Conclusions: Our preliminary data demonstrates modest but successful transfection of patient iPSC-derived organoid cultures ‘in situ’ in 3D matrigel using a commercial transfection reagent. Further development of these methods will facilitate miRNA:Target gene interaction studies to complement ongoing research in iPSC organoid platforms and inform delivery of future therapeutic strategies utilising small RNA.

Source: European Respiratory Journal - December 1, 2022 Category: Respiratory Medicine Authors: Oglesby, I., De Santi, C., Schweikert, A., Cryan, S. A., Hurley, K. Tags: 03.02 - Airway cell biology and immunopathology Source Type: research