Characterization of neutral lipases revealed the tissue ‐specific triacylglycerol hydrolytic activity in Nilaparvata lugens

Forty-three lipases inNilaparvata lugens were identified, with half of them belonging to neutral lipases. The neutral lipases inN.  lugens intestine lost triacylglycerol hydrolytic activity and ovary-specific neutral lipases played important roles in lipid metabolism and the reproduction ofN.  lugens. AbstractThe lipid metabolism plays an essential role in the development and reproduction of insects, and lipases are important enzymes in lipid metabolism. InNilaparvata lugens, an important insect pest on rice, triacylglycerol hydrolytic activities were different among tissues, with high activity in integument, ovary, and fat body, but low activity in intestine. To figure out the tissue-specific triacylglycerol hydrolytic activity, we identified 43 lipases inN.  lugens. Of these 43 lipases, 23 belonged to neutral lipases, so this group was selected to perform further experiments on triacylglycerol hydrolysis. The complete motifs of catalytic triads,β9 loop, and lid motif, are required for the triacylglycerol hydrolytic activity in neutral lipases, which were found in some neutral lipases with high gene expression levels in integument and ovary, but not in intestine. The recombinant proteins of 3 neutral lipases with or without 3 complete motifs were obtained, and the activity determination confirmed the importance of 3 motifs. SilencingXM_022331066.1, which is highly expressed in ovary and with 3 complete motifs, significantly decreased the egg production and hatchability o...
Source: Insect Science - Category: Biology Authors: Tags: ORIGINAL ARTICLE Source Type: research