Comprehensive reparative effects of bacteriostatic poly(L ‐lactide‐co‐glycolide)/poly(L‐lactide‐co‐ε‐caprolactone) electrospinning membrane on alveolar bone defects in progressive periodontitis

In this study a serial clinically applied antibiotics loaded poly(L-lactide-co-glycolide)/poly(L-lactide-co- ε-caprolactone) (PLGA/PLCA) fibrous mesh to prevent and reconstruct defective bone in periodontitis were prepared by electrospinning. Incorporation of antibiotics promoted the hydrophilicity but decreased the crystallinity of PLGA/PLCA membranes. Antibiotics could be sustained released from membran es. Metronidazole, minocycline, and doxycycline incorporated membranes could suppressPorphyromonas gingivalis (P. gingivalis) within 21  days in vitro. Metronidazole and minocycline incorporated membranes decreased 41% and 55.5% colony counts in rat gingival crevicular fluid in vivo. Minocycline-loaded membrane could support the proliferation of MC3T3-E1 cells and maintained 79% viability of human ligament fibroblasts cultured on it. And MC3T3-E1 cells could undergo osteoblastic differentiation when cultured with pure PLGA/PLCA membrane and minocycline incorporated membrane. Then in vivo repairable effects of those antibiotics loaded membranes were evaluated in alveolar bone defectedP. gingivalis infected model. The application of minocycline loaded membranes, effectively prevented the bone resorption of periodontitis caused byP. gingivalis. After been treated with minocycline incorporated membrane, volume of defected bone of maintained at about 50% level of control rats. 8  weeks post-operation, newly regenerated bone was observed in the operative alveolar bone of the ...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - Category: Materials Science Authors: Tags: RESEARCH ARTICLE Source Type: research