Effect of cooking duration on the antioxidant and antihyperglycemic properties of Andrographis paniculata (king of bitters) leaves in high sucrose diet-fed fruit flies (Drosophila melanogaster)

This study evaluated the effects of cooking duration on the antioxidant and antihyperglycemic properties ofAndrographis paniculata on high sucrose diet fed fruit flies (Drosophila melanogaster).A. paniculata leaves were pulverized and divided into three portion; one was subjected to water infusion and the other two portions were cooked for 5 and 10  min respectively. Wild-typeD. melanogaster were raised for 14  days on high sucrose diet (HSD) supplemented withA. paniculata at different cooking intervals. Thereafter, the mortality rate, negative geotaxis, body weight, antioxidant indices, thiobarbituric reactive substances (TBARS), total thiol, superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST), and carbohydrate metabolism indices (total glucose, α-amylase, α-glucosidase) were determined. The result also revealed that the flies fed with HSD diet showed significant reductions in negative geotaxis accompanied by elevation in mortality rate, TBARS level, glucose concentration, and α-amylase and α-glucosidase activities, as well as reduction s in total thiol level, SOD, catalase, and GST activities. However, flies fedA. paniculata supplemented diet significantly reversed the observed impairments with 5-min cooking time exhibiting the best antihyperglycemic and antioxidant effects. Varied amounts of gallic acid, catechin, ferulic acid, rutin, quercetin, and kaempferol were identified and quantified via HPLC analysis in the uncooked and cooked samples....
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research