Glioma 2021 WHO Classification: The Superiority of NGS Over IHC in Routine Diagnostics

ConclusionsWe demonstrated that qPCR or NGS performed in reference genetic laboratories, rather than IHC, is the most reliable method forIDH1/2 analysis. Clinicians should be aware of discrepancies in MLPA or IHC results and seek reconsultation in facilities with extensive access to advanced molecular technologies. Moreover, we proposed a new algorithm for the molecular diagnostic procedures in glioma patients based on the WHO CNS5.Graphical abstract
Source: Molecular Diagnosis and Therapy - Category: Molecular Biology Source Type: research